Deleted
Sample Discontinued
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Coding

Part:BBa_K2139000

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-13)


Coding Sequence of Endo5a

BBa_K2139000 encompass the coding region for a endo-beta-1,4-glucanase known as Endo5A. Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature of 50 degrees Celsius and pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid) ,and a PDB code of 1VRX. This construct was synthesized for use in Caulobacter Cresentus and as such as codon optimized to contain a high GC content.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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